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KMID : 0603820070130040293
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Journal of Experimental & Biomedical Science
2007 Volume.13 No. 4 p.293 ~ p.304
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Patterns of Antimicrobial Resistance and Genotyping of Extended Spectrum $\beta$-Lactamase (ESBL) Producing Clinical Isolates in Korea
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Lee Gyu-Sang
Kim Jong-Bae
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Abstract
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The emergence of extended spectrum beta-lactamase (ESBL) producing bacteria is worldwide concern. Until recently, the most frequently identified strains in the Republic of Korea were E. coli and Klebsiella spp. The incidence of resistance to extended spectrum beta-lactam antibiotics is increasing in Wonju city, Korea. Total 57 strains of ESBL producing E. coli and Klebsiella species were isolated from Wonju Christian Hospital during a 9 month-period from April to December, 2003. To determine the prevalence and genotypes of the ESBL producing clinical isolates, antibiotic susceptibility and ESBL activity test by VITEK system and double disk synergy (DDS) test, and PCR based genotyping were performed. Fourteen (82%) isolates of 17 ESBL producing E. coli were found to have bla_{TEM} gene and 5 (29%) isolates were found to have bla_{CTX-M} gene by polymerase chain reaction (PCR). Thirty (75%) isolates of 40 ESBL producing Klebsiella species with bla_{TEM} gene, 38 (95%) isolates with bla_{SHV} gene, and 7 (20%) isolates with bla_{CTX-M} type gene were also identified. Enterobacterial repetitive intergenic consensus (ERIC) PCR and similarity index by dendrogram for genetical similarity to band pattern of each clinical isolates were examined. ESBL producing E. coli were grouped into 6 clusters up to 84% of similarity index and Klebsiella species were grouped into 12 clusters up to 76% of similarity index. In conclusion, ESBL producing clinical isolates were characterized with the results from antimicrobial resistance pattern and genetical similarity using ERiC PCR.
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KEYWORD
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Extended spectrum beta-lactamase (ESBL), Polymerase chain reaction (PCR), Enterobacterial repetitive intergenic consensus (ERIC)
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